The Miami Project’s Core Facilities
Offerings at the Core Facilities
The Miami Project to Cure Paralysis
Lois Pope LIFE Center Building
University of Miami Miller School of Medicine
TRANSMISSION ELECTRON MICROSCOPY CORE FACILITY
The University of Miami Miller School of Medicine has had a long-standing tradition of providing quality fee-based electron microscopy service to the medical school campus. The Transmission Electron Microscopy (TEM) Core operates under the aegis of The Miami Project to Cure Paralysis. The tissue processing laboratory can be found on the 5th floor of the Lois Pope Life building (Room 5-23/24). The TEM Core maintains two transmission electron microscopes located in the Lois Pope Life Center (Room 4-34): a JEOL JEM-1400 and a Philips CM-10. In the TEM Core, we are able to handle many types of tissue and use several types of tissue preparations, including block tissue, tissue cultures, cell pellets and exosomes. Embedding immunostained tissue and tissue for immunogold staining is also provided.
HIGH CONTENT CORE FACILITY
Using the ThermoFisher Cellomics ArrayScan VTI instruments and associated automated cellular analysis software, the High Content Screening (HCS) Core provides a cutting edge, multiplexed functional screening and imaging platform to perform multi-well cell-based assays. Read more…
Yan Shi, M.S.
HISTOLOGY CORE FACILITY
Histology Core Facility The Histology Core provides a full range of histology services. These include paraffin and frozen tissue processing, embedding, sectioning and routine staining. The Core is staffed by five experienced technicians available to assist researchers with their histology needs. The facility also houses two Leica crystostats, a freezing stage microtome and vibratome which are readily available to researchers.
Yoelvis Hernandez Rodriguez
MICROSCOPY CORE FACILITY
The Imaging Core facility at The Miami Project to Cure Paralysis provides a wide range of microscopy and imaging instrumentation to Miami Project researchers and collaborators. The Core facility maintains brightfield and fluorescent microscope workstation equipped with MicroBrightField stereology analysis software and a laser scanning confocal system with spectral unmixing capability. Notably, the Core houses the advanced LaVision Ultramicroscope and a dedicated Imaris rendering workstation. As a full-service facility, our aim is to provide scientific and technical expertise in all aspects of imaging, from proper sample preparation, data acquisition at the microscope, to processing and analysis of image data.
Yan Shi, M.S.