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VIRAL VECTOR CORE FACILITY

The Miami Project to Cure Paralysis
Lois Pope LIFE Center Building Room 4-26
Phone: (305) 243-0407
Please email questions to: VVC@med.miami.edu

CORE DIRECTORS

Kevin Park, Ph.D.
Pantelis Tsoulfas, M.D.

CORE MANAGER

Yania Martinez, BSc.
305-243-7123

The Viral Vector Core (VVC) is open to all University of Miami researchers. The VVC specializes in producing lentiviral and adeno-associated virus (AAV) viral particles, as well as other types of viral particles (e.g., Adenovirus, FIV).  The VVC produces high-titer lenitviral and AAV viral particles at lower prices compared to commercial sources. Furthermore, using the VVC will likely provide a significant cost savings versus an individual lab trying to produce the viral particles on its own (consider the costs of reagent, labor, time, and troubleshooting).

For generating custom viral particles, the investigator provides the VVC with a high-quality maxiprep of the transfer plasmid to be packaged into the viral particle of choice.  Turn-around time is about 2 weeks for lentivrus and AAV viral particles.

NOTE: You must have IBC approval to obtain viral particles.

This is often the rate-limiting step in obtaining viral particles, and we are not legally allowed to give out viral particles without this. The approval form can be found on the University of Miami Institutional Biosafety Committee website.

 

VIRAL VECTOR OFFERINGS VOLUME TYPICAL TITER PRICE FOR UM USERS PRICE FOR EXTERNAL USERS
Ultra-purified Lentivirus 200 μl of virus* (customized aliquoting) 2×107– 3×108 pg/ml $850.00 $1,304.00
Aliquot stock of ultra-purified
Lentivirus
20 μl/vial 2×107– 3×108 pg/ml $95.00 $145.00
Ultra-purified  AAV 100 μl of virus*  (customized aliquoting)  >2×1013GC/ml** $1,112.00 $1,706.00
Standard AAV preparation 100 μl of virus*  (customized aliquoting) >2×1013 GC/ml** $995.00 $1,527.00
Aliquot stock of AAV 10 μl/vial >2×1013GC/ml** $129.00 $198.00
Maxiprep plasmid preparation for making a virus N/A N/A $252.00 $386.60

* Higher volume of viruses are provided upon request at equivalent prices.

** Titers may vary depending on the AAV serotype and transgene used.

AAV CONTROL STOCKS
AAVs of various serotypes with different promoters and reporters are available.
Promoters:   UBC, CMV, CAG.
Serotypes:    AAV1, AAV2, AAV5, AAV8, AAV9, AAV2-retro, AAV PHP.S and AAV PHP.eB
Reporters:    Cre, GFP, TdTomato, mCherry, mGreenLantern, mNeonGreen, mScarlet-I, Placental alkaline phosphatase (PLAP).

LENTIVIRUS CONTROL STOCKS
Pseudotyped with VSVG
Promoters:   CMV
Reporters:    GFP, mCherry, LacZ, mScarlet-I, Azzurite

ORDER FORMS

PLASMIDS MAPS

Customer testimonials

“My laboratory used the VVC to produce recombinant adeno-associated vector (AAV) preparations for our vision research. The VVC team has provided excellent service and high quality and high titer AAV vectors.” Dmitry Ivanov, PhD.

“The Viral Vector Core is invaluable! Their technical support, timely handling of orders and excellent quality of virus preparations are outstanding. They helped my lab develop new tools, new research directions, and likely, new funded projects.” Nirupa Chaudhari, PhD.

PUBLICATIONS SHOWCASING OUR VIRAL VECTORS

• Mah KM, Wu W, Al-Ali H, Sun Y, Han Q, Ding Y, Muñoz M, Xu XM, Lemmon VP, Bixby JL. Compounds co-targeting kinases in axon regulatory pathways promote regeneration and behavioral recovery after spinal cord injury in mice. Exp Neurol. 2022 May 16;355:114117. doi: 10.1016/j.expneurol.2022.114117. Epub ahead of print. PMID: 35588791.

• Asencor AI, Dvoryanchikov G, Makhoul V, Tsoulfas P, Chaudhari N. Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro. 2022 Jun 6;9(3):ENEURO.0373-21.2022. doi: 10.1523/ENEURO.0373-21.2022. PMID: 35610024; PMCID: PMC9186415.

• Makhmutova M, Weitz J, Tamayo A, Pereira E, Boulina M, Almaça J, Rodriguez-Diaz R, Caicedo A. Pancreatic β-Cells Communicate With Vagal Sensory Neurons. Gastroenterology. 2021 Feb;160(3):875-888.e11. doi: 10.1053/j.gastro.2020.10.034. Epub 2020 Oct 26. PMID: 33121946.

• Lowell JA, Mah KM, Bixby JL, Lemmon VP. AAV8 transduction capacity is reduced by prior exposure to endosome-like pH conditions. Neural Regen Res. 2021 May;16(5):851-855. doi: 10.4103/1673-5374.299272. PMID: 33229719; PMCID: PMC8178773.

• Pearse DD, Rao SNR, Morales AA, Wakarchuk W, Rutishauser U, El-Maarouf A, Ghosh M. Engineering polysialic acid on Schwann cells using polysialyltransferase gene transfer or purified enzyme exposure for spinal cord injury transplantation. Neurosci Lett. 2021 Mar 23;748:135690. doi: 10.1016/j.neulet.2021.135690. Epub 2021 Feb 1. PMID: 33540059.

• Yadav S, Yuan CC, Kazmierczak K, Liang J, Huang W, Takeuchi LM, Kanashiro-Takeuchi RM, Szczesna-Cordary D. Therapeutic potential of AAV9-S15D-RLC gene delivery in humanized MYL2 mouse model of HCM. J Mol Med (Berl). 2019 Jul;97(7):1033-1047. doi: 10.1007/s00109-019-01791-z. Epub 2019 May 17. PMID: 31101927; PMCID: PMC6584042.

• Wang Z, Mehra V, Simpson MT, Maunze B, Chakraborty A, Holan L, Eastwood E, Blackmore MG, Venkatesh I. KLF6 and STAT3 co-occupy regulatory DNA and functionally synergize to promote axon growth in CNS neurons. Sci Rep. 2018 Aug 22;8(1):12565. doi: 10.1038/s41598-018-31101-5. PMID: 30135567; PMCID: PMC6105645.

• Venkatesh I, Mehra V, Wang Z, Califf B, Blackmore MG. Developmental Chromatin Restriction of Pro-Growth Gene Networks Acts as an Epigenetic Barrier to Axon Regeneration in Cortical Neurons. Dev Neurobiol. 2018 Oct;78(10):960-977. doi: 10.1002/dneu.22605. Epub 2018 Jun 14. PMID: 29786967; PMCID: PMC6204296.

• Al-Ali H, Ding Y, Slepak T, Wu W, Sun Y, Martinez Y, Xu XM, Lemmon VP, Bixby JL. J Neurosci. 2017 Jul 26;37(30):7079-7095. doi: 10.1523/JNEUROSCI.0931-17.2017. Epub 2017

• Zhu Y, Lyapichev K, Lee DH, Motti D, Ferraro NM, Zhang Y, Yahn S, Soderblom C, Zha J, Bethea JR, Spiller KL, Lemmon VP, Lee JK. J Neurosci. 2017 Mar 1;37(9):2362-2376. doi: 10.1523/JNEUROSCI.2751-16.2017. Epub 2017 Jan 27.

• Bray ER, Noga M, Thakor K, Wang Y, Lemmon VP, Park KK, Tsoulfas P. 3D Visualization of Individual Regenerating Retinal Ganglion Cell Axons Reveals Surprisingly Complex Growth Paths. eNeuro. 2017 Aug 29;4(4):ENEURO.0093-17.2017. doi: 10.1523/ENEURO.0093-17.2017. PMID: 28856242; PMCID: PMC5575138.

• Jergova S, Gordon CE, Gajavelli S, Sagen J. Experimental Gene Therapy with Serine-Histogranin and Endomorphin 1 for the Treatment of Chronic Neuropathic Pain. Front Mol Neurosci. 2017 Dec 8;10:406. doi: 10.3389/fnmol.2017.00406. PMID: 29276474; PMCID: PMC5727090.

• Luo X, Ribeiro M, Bray ER, Lee DH, Yungher BJ, Mehta ST, Thakor KA, Diaz F, Lee JK, Moraes CT, Bixby JL, Lemmon VP, Park KK. (2016). Enhanced transcriptional activity and mitochondrial localization of STAT3 co-induce axon regrowth in the adult central nervous system. Cell Reports. 15(2):398-410.

• Haenzi B, Gers-Barlag K, Akhoundzadeh H, Hutson TH, Menezes SC, Bunge MB, Moon LD. (2016). Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. PLoS ONE. 11(3):e0150541.

• Haenzi B, Gers-Barlag K, Akhoundzadeh H, Hutson TH, Menezes SC, Bunge MB, Moon LD. (2016). Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. PLoS ONE. 11(3):e0150541.

• Dvoriantchikova G, Pappas S, Luo X, Ribeiro M, Danek D, Pelaez D, Park KK, Ivanov D. (2016). Virally delivered, constitutively active NFκB improves survival of injured retinal ganglion cells. European Journal of Neuroscience. 44(11):2935-2943.

• Dvoriantchikova G, Pappas S, Luo X, Ribeiro M, Danek D, Pelaez D, Park KK, Ivanov D. (2016). Virally delivered, constitutively active NFκB improves survival of injured retinal ganglion cells. European Journal of Neuroscience. 44(11):2935-2943.

• Jergova S, Gajavelli S, Pathak N, Sagen J. (2016). Recombinant neural progenitor transplants in the spinal dorsal horn alleviate chronic central neuropathic pain. Pain. 157(4):977-989.

• Hackett AR, Lee DH, Dawood A, Rodriguez M, Funk L, Tsoulfas P, Lee JK. (2016). STAT3 and SOCS3 regulate NG2 cell proliferation and differentiation after contusive spinal cord injury. Neurobiology of Disease. 89:10-22.

• Mehta ST, Luo X, Park KK, Bixby JL, Lemmon VP. (2016). Hyperactivated Stat3 boosts axon regeneration in the CNS. Experimental Neurology. 280:115-120.