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Core Facility: High Content Screening

About the High Content Screening Facility

The Miami Project to Cure Paralysis
Lois Pope LIFE Center Building Room 4-26A

Phone: (305) 243-6793
Please email questions to: YSHI@med.miami.edu

Core DirectorS
Vance Lemmon, Ph.D.
John Bixby, Ph.D.
305-243-6793





Using the ThermoFisher Cellomics ArrayScan VTI and PerkinElmer OPERA high content screening systems and associated automated cellular analysis softwares, the Janus liquid handling robot, and the Molecular Devices Spectramax i3X plate reader, the High Content Screening (HCS) Core provides a cutting edge, multiplexed functional screening and imaging platform to perform multi-well cell-based and target-based assays.

SERVICES AND EXPERTISE
Technical Consultation Service

COSTS
$ 75 per hour for University of Miami users.
$ 120 per hour for non-UM (external) users.

Provides expertise and know how specific to the successful planning and performance of customers’ High Content Screening projects. For UM faculty, the initial consultation of up to 2 hours is free.

HCS Setup Fee
$ 90 per hour for University of Miami users.
$ 135 per hour for non-UM (external) users.

This service charge includes staff and machine time. This service charge is associated with setting up a test plate prior to the first experimental run. The service involves configuring appropriate machine parameters and software settings to optimize image acquisition and generate reliable and relevant output/data.

Batch Run Setup Fee
$ 80 per hour for University of Miami users.
$ 120 per hour for non-UM (external) users.

This service charge includes staff and machine time. This service charge is associated with setting up the HCS machine to run a batch of plates. Before running a batch of plates, qualification of the initial plate is conducted to ensure that previously determined settings and parameters are still appropriate for the batch of plates. This ensures the batch run will result in good output.

HSC Instrument Fee
$ 45 per hour for University of Miami users (min. charge $10).
$ 70 per hour for non-UM (external) users (min. charge $12.50).

This service charge includes machine time for running the plates. Minimum charge is fifteen (15) minutes. The charge is a function of number of plates and the time it takes to run each plate.

Liquid Handling Instrument Fee
$ 100 per hour for University of Miami users (min. charge $25).
$ 155 per hour for non-UM (external) users (min. charge $39).

This service charge includes machine time for running the plates. Minimum charge is fifteen (15) minutes. The charge is a function of number of plates and the time it takes to run each plate.

Liquid Handling Instrument Setup Fee
$ 150 per hour for University of Miami users.
$ 230 per hour for non-UM (external) users.

The service involves personnel setting up the Liquid handling Instrument Protocol.  It is worth noting that programing and testing the robot for a liquid handling project, such as rearranging and diluting a large compound library for a screen, is non-trivial and can take a few days.  As the libraries are usually precious and available in limited quantities, this should not be surprising.

Imaging Processing and Analysis
$ 75 per hour for University of Miami users.
$ 120 per hour for non-UM (external) users.

This service charge is for personnel time involved in  processing the large data files generated after running the customers plates. Each plate generates data that is reviewed for quality control purposes.

Columbus Image Analysis System
$ 60 per hour for University of Miami users.
$ 95 per hour for non-UM (external) users.

The charge is a function of number of individual plates or runs submitted to the server.

Plate Reader
$ 83 per hour for University of Miami users (min charge $22).
$ 130 per hour for non-UM (external) users (min charge $33).

This service charge includes machine time for running the plates. Minimum charge is fifteen (15) minutes. The charge is a function of number of plates and the time it takes to run each plate.

 

FREQUENTLY ASKED QUESTIONS

1. When can I expect to get my results back?
Once the run is scheduled, the High Content Screening results will be delivered within a week, although in many cases the results will be available in 24-48 hours.

2. What do I need to provide for High Content Screening?
Please fill out the HCS Image Facility Request Form before you plan your experiment using the High Content Screening facility. The ArrayScan VTI and Kinetic Scan are capable of scanning 6 well/12 well/24well/48well/96 well plates as well as coverslips. We recommend Falcon and Packard plates. Other types of plates may be allowed but will required additional setup time for new form factors, at an increased expense.  Please consult the facility manager before settling on a plate or coverslip arrangement.  Please check staining/fluorescence quality before you bring your plates to the HCS core to insure a successful screening.

3. In what form are the results?
The results of High Content Screening are provided in Excel format. The HCS Explorer allows the user to access the images to ensure the quality of the samples. We will gladly help the user in interpretation of the results.  As an option, we can use Spotfire DecisionSite software to perform analysis the data and provide PDF or PowerPoint file of the results and email them to the user.

Instructions are available for filling out the IDR Form.

4. How do I pay for use of the facility?
Payment for services is through an IDR (Interdepartmental Requisition). Please fill out an IDR and deliver it to Yan Shi on the Medical Campus using locator code R-48 and LPLC as the building and 4-25 for the room number. We will fill out the amount to be charged based on the actual service time after we have finished the screening and email the user to pick up their copy.

5. Who is eligible to use the facility?
The HCS Core will be open to all interested customers for a wide range of research projects. University users will have priority.