LOCUS pLenti-MP2-MCS-I 8206 bp DNA circular 5-SEP-2007 SOURCE ORGANISM COMMENT Created by Pingping Jia and Anthony A. Oliva, Ph.D. COMMENT ORIGDB|GenBank COMMENT VNTDATE|456638735| COMMENT VNTDBDATE|456661448| COMMENT LSOWNER| COMMENT VNTNAME|pLenti-MP2-MCS-IRES2-EGFP| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers CDS 615..1334 /gene="EGFP" /product="Enhanced green fluorescent protein open reading frame" /SECDrawAs="Gene" /vntifkey="4" /label=EGFP CDS 27..611 /gene="IRES2" /product="Internal ribosome entry site (IRES) Clontech version #2" /SECDrawAs="Gene" /vntifkey="4" /label=IRES2 CDS 4537..5210 /gene="Ori" /product="pUC origin of replication" /SECDrawAs="Gene" /vntifkey="4" /label=Ori CDS 3402..4392 /gene="Amp" /product="Ampicillin resistance gene cassette" /SECDrawAs="Gene" /vntifkey="4" /label=Amp CDS 2946..3401 /gene="f1" /product="f1 origin of replication" /SECDrawAs="Gene" /vntifkey="4" /label=f1 CDS 2584..2743 /gene="SV40 promoter" /product="SV40 early promoter (partial)" /SECDrawAs="Gene" /vntifkey="4" /label=SV40\promoter CDS 2463..2574 /gene="polyA" /product="SV40 polyadenylation signal (partial)" /SECDrawAs="Gene" /vntifkey="4" /label=polyA misc_feature 2443..2573 /gene="SV40 pA" /product="SV40 polyadenylation signal according to Invitrogen" /SECDrawAs="Region" /vntifkey="21" /label=SV40\pA misc_feature 2191..2371 /gene="LTR" /product="Truncated HIV-1 3' LTR" /SECDrawAs="Region" /vntifkey="21" /label=LTR misc_feature 2137..2190 /gene="delta U3" /product="delta U3" /SECDrawAs="Region" /vntifkey="21" /label=delta\U3 CDS 2137..2371 /gene="LTR" /product="delta U3 3' LTR" /SECDrawAs="Gene" /vntifkey="4" /label=LTR CDS 1463..2054 /gene="WPRE (1093-1684)" /product="woodchuck hepatitis virus posttranscriptional regulatory element" /SECDrawAs="Gene" /vntifkey="4" /label=WPRE CDS 7551..8135 /gene="hCMV E/P" /product="Human cytomegalovirus immediate early gene enhancer, promoter, & leader" /SECDrawAs="Gene" /vntifkey="4" /label=hCMV\E/P CDS 6692..6925 /gene="RRE" /product="HIV-1 Rev response element" /SECDrawAs="Gene" /vntifkey="4" /label=RRE CDS 6138..6182 /gene="Psi" /product="HIV-1 psi packaging signal" /SECDrawAs="Gene" /vntifkey="4" /label=Psi misc_feature 5847..6027 /gene="LTR" /product="HIV-1 5' LTR" /SECDrawAs="Region" /vntifkey="21" /label=LTR CDS 5618..6027 /gene="LTR" /product="RSV 5' LTR hybrid promoter" /SECDrawAs="Gene" /vntifkey="4" /label=LTR misc_feature 5618..5846 /gene="RSV promoter" /product="RSV promoter" /SECDrawAs="Region" /vntifkey="21" /label=RSV\promoter BASE COUNT 2110 a 2040 c 2073 g 1983 t ORIGIN 1 tcgacggtac cgcgggcccg ggatccgccc ctctccctcc ccccccccta acgttactgg 61 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 121 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 181 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 241 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 301 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 361 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 421 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 481 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 541 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 601 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 781 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 1021 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 1081 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 1141 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 1201 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 1261 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 1321 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga 1381 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 1441 tgcaattgtt gttgttatcg acaatcaacc tctggattac aaaatttgtg aaagattgac 1501 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 1561 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 1621 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 1681 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 1741 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 1801 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 1861 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 1921 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 1981 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 2041 cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta caaggcagct 2101 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 2161 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 2221 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 2281 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 2341 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 2401 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 2461 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2521 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 2581 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 2701 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 2761 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 2821 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 2881 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 2941 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3001 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3061 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3121 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3181 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3241 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 3301 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3361 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 3421 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3481 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3541 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3601 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3661 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3721 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3781 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3841 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3901 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3961 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4021 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4081 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4141 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4201 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4261 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4321 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4381 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4681 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 4741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4921 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5221 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 5281 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 5341 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5401 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 5461 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 5521 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 5581 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct 5641 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 5701 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac 5761 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat 5821 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg 5881 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 5941 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6001 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 6061 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 6121 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 6181 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 6241 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 6301 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 6361 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 6421 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 6481 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 6541 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 6601 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 6661 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 6721 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 6781 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 6841 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 6901 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 6961 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 7021 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 7081 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 7141 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 7201 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 7261 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 7321 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 7381 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga 7441 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 7501 aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg ggagttccgc 7561 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 7621 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 7681 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 7741 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 7801 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 7861 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 7921 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 7981 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 8041 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc 8101 catccacgct gttttgacct ccatagaaga caccgcccgg gcgtacggga tcccaccggt 8161 gctcgagatg catactagtg aattcacgcg ttctagacct gcaggg //