LOCUS pAAV-EGFP-WPRE 5983 bp DNA circular SYN 15-JAN-2007 DEFINITION pAAV-EGFP, complete sequence. ACCESSION AF396260 VERSION AF396260.1 GI:15148786 SOURCE Cloning vector pAAV-MCS. ORGANISM Cloning vector pAAV-MCS other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4650) AUTHORS Youngblood,G. and Grafsky,A.J. TITLE Direct Submission JOURNAL Submitted (29-JUN-2001) Technical Services, Stratagene, 11011 N Torrey Pines Rd, La Jolla, CA 92037, USA COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|435431499| COMMENT VNTDBDATE|435431499| COMMENT LSOWNER| COMMENT VNTNAME|pAAV-EGFP-WPRE| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers repeat_region 1..141 /rpt_type=inverted /vntifkey="34" /label=L-ITR /note="left AAV-2ITR" promoter 150..812 /vntifkey="30" /label=P\CMV /note="CMV" repeat_region 3246..3386 /rpt_type=inverted /vntifkey="34" /label=R-ITR /note="right AAV-2ITR" rep_origin 3461..3921 /vntifkey="33" /label=f1\ori /note="f1" CDS 4303..5163 /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAK84839.1" /db_xref="GI:15148787" /vntifkey="4" /label=ampicillin /note="ampicillin resistance" rep_origin 5175..5983 /vntifkey="33" /label=pUC\ori /note="ColE1" polyA_signal 2728..3206 /vntifkey="25" /label=hGH\pA /note="hGH polyA" intron 820..1312 /vntifkey="15" /label=beta-globin\intron /note="¦Â-globin intron" misc_feature 1373..2084 /vntifkey="21" /label=EGFP misc_feature 2091..2682 /vntifkey="21" /label=WPRE BASE COUNT 1336 a 1631 c 1513 g 1503 t ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt ccccggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 1381 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 1441 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 1501 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 1561 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 1621 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 1681 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 1741 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 1801 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 1861 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 1921 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 1981 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2041 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc 2101 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 2161 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 2221 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 2281 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 2341 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 2401 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 2461 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 2521 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 2581 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 2641 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggcttg 2701 cctcgagcag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc 2761 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa 2821 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg 2881 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac 2941 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag 3001 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca 3061 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca 3121 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt 3181 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg 3241 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 3301 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 3361 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct 3421 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca 3481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 3541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 3601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 3661 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt 3721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3781 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg 3841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 3901 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 3961 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 4021 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 4081 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 4141 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 4201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 4321 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4381 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4441 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4501 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4561 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4621 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4681 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4741 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4801 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4861 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4921 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 4981 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 5041 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 5101 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 5161 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 5221 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5461 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt //